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Titlebook: Antiviral Resistance in Plants; Methods and Protocol John M. Watson,Ming-Bo Wang Book 2012 Springer Science+Business Media, LLC 2012 Antivi

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發(fā)表于 2025-3-21 18:25:32 | 只看該作者 |倒序瀏覽 |閱讀模式
期刊全稱Antiviral Resistance in Plants
期刊簡稱Methods and Protocol
影響因子2023John M. Watson,Ming-Bo Wang
視頻videohttp://file.papertrans.cn/159/158709/158709.mp4
發(fā)行地址Details many of the methods currently used to study RNA silencing in relation to viral infections in plants.Features step-by-step, easy to follow protocols from an international assortment of laborato
學科分類Methods in Molecular Biology
圖書封面Titlebook: Antiviral Resistance in Plants; Methods and Protocol John M. Watson,Ming-Bo Wang Book 2012 Springer Science+Business Media, LLC 2012 Antivi
影響因子.Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In .Antiviral Resistance in Plants: Methods and Protocols., expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants.? These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes.? Written in the highly successful .Methods in Molecular Biology?. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troublesho
Pindex Book 2012
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Strategies for Viral Cross Protection in Plants,the mild virus strain to establish MSCP. To investigate interference among virus strains and the plant host at an early stage of MSCP at a subcellular level, we developed a rapid micro-extraction method for the preparation of total nucleic acid (TNA), combined with other molecular methods, to monito
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Plant Viroids: Isolation, Characterization/Detection, and Analysis,red approximately 10 years later, and much effort has subsequently been devoted to simplifying the sample preparation procedure and identifying group-specific primer pairs. This chapter describes four simple, easy-to-follow protocols—two involving PAGE and two others based on enzymatic amplification
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Peter Kuhn,Matthias Buchinger,Dian Balta gene regulation systems. From the very beginning, plant research has had a major impact on our understanding of RNAi. The purpose of this chapter is to provide an historical perspective and overview on the discovery, characterization, and applications of RNAi in plants.
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Lecture Notes in Computer Scienceornamental and plant biotechnology, vol. III, 2006). Four distinct RNA silencing pathways have been recognized in plants, albeit with considerable conservation of the molecular components. These pathways are directed by various small RNA species, including microRNAs (miRNAs), .-acting small interfer
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