Titlebook: Wheat Biotechnology; Methods and Protocol Prem L. Bhalla,Mohan B. Singh Book 2017 Springer Science+Business Media LLC 2017 Biotechnological

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Doubled Haploid Laboratory Protocol for Wheat Using Wheat–Maize Wide Hybridizationral generations to reach homozygosity in loci controlling traits of interest. Using doubled haploid technology, however, it is possible to attain 100% homozygosity at all loci in a single generation and completely homogeneous breeding lines can be obtained in 1–2?years. Thus, doubled haploid technol

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Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Brea. Development and validation of suitable ERGs is the primary step for establishing assays that monitoring the genetically modified (GM) contents in food/feed samples. Herein, we give a review of the ERGs currently used for GM wheat analysis, such as ., ., ., and ., as well as their performances in G

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Biolistic Transformation of Wheatpter, we describe a protocol for biolistic transformation of wheat that uses immature embryos and small quantities of DNA cassettes. An original method for DNA cassette purification is also described. This protocol can be used to transform a wide range of wheat genotypes and other related species.

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Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Breaod/feed samples. Herein, we give a review of the ERGs currently used for GM wheat analysis, such as ., ., ., and ., as well as their performances in GM wheat analysis. Also, we discussed one model for developing and validating one ideal RG for one plant species based on our previous research work.

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Rohit Joshi,Khalid Anwar,Priyanka Das,Sneh L. Singla-Pareek,Ashwani Pareekng these features it was found expedient to introduce the concept of an aspect specification for describing properties of entities in general. It is thus convenient to describe aspect specifications in this chapter.

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ed. Adjustments are having to be made. In Northern Ireland, as elsewhere, the removal of opportunities to enter skilled employment and the occupational culture it provides, has threatened long-established social and gender identities. In turn new ‘solutions’ to address this crisis in cultural reproduction are being explored by young Loyalists.