Titlebook: Wheat Biotechnology; Methods and Protocol Prem L. Bhalla,Mohan B. Singh Book 2017 Springer Science+Business Media LLC 2017 Biotechnological

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-Mediated Transformation of Wheat Using Immature Embryoscies between 5 and 15%. Whereas primary transgenic plants regenerated in vitro commonly show reduced fitness and fertility, no apparent variations with regard to morphology and grain set in their transgenic progeny as compared to wild-type counterparts were observed.

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Design and Assembly of CRISPR/Cas9 Reagents for Gene Knockout, Targeted Insertion, and Replacement iirus replicons (GVRs). The reagents allow for both easy construction of simple genome engineering vectors, and “mix and match” swapping of components such as the Cas9, guide RNA and donor template cassettes for gene targeting. In addition, a web-based tool greatly streamlines vector selection, primer design, and vector construction.

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Phenotypic Characterization of Transgenic Wheat Lines Against Fungal Pathogens , and , defensin also provides Type II resistance to FHB under controlled growth chamber conditions. These findings have practical applications for control of these important fungal diseases in wheat. Here, we provide details on conducting LR and FHB bioassays of transgenic wheat lines in the growth chamber.

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