Titlebook: Lipidomics; Methods and Protocol Sanjoy K. Bhattacharya Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under excl

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High-Performance Chromatographic Separation of Cerebrosides,, we describe the analytical separation of glucosylceramide and galactosylceramide by HPTLC. This technique can be used for quantitation purposes but also with small modification for subsequent mass spectrum analysis for structural determination.

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Analysis of Lipids, Fatty Acid, and Cholesterol in Membrane Microdomains,ization of such components into discrete membrane microdomains (known as .). The lipid microdomain hypothesis has generated significant controversy and rigorous inquiry to test the idea that such domains concentrate machinery to mediate cellular processes such as signaling, synaptic plasticity, and

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Analyses and Localization of Phosphatidylcholine and Phosphatidylserine in Murine Ocular Tissue Usiounds through mass spectrometry, combined with mapping the compound distribution in the sample in a targeted or untargeted approach, renders IMS a powerful tool for lipidomics. IMS analysis for lipid species such as phosphatidylcholine and phosphatidylserine allows researchers to pinpoint areas of l

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A Robust Phenotypic Screening Assay Utilizing Human Podocytes to Identify Agents that Modulate Lipite and behavior. In recent years, their relevance as therapeutic targets for a wide array of human diseases has been well established. Consequently, efforts to develop tools to study them have intensified, including assays that can accurately track LD levels in clinically relevant cell-based models.

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Capillary Electrophoresis Plasma Fractionation for Lipids Analysis, specificity. Complex mixtures like blood plasma contain multiple lipid classes, whose distribution in the body are mediated by protein–lipid interactions and integration of lipids in larger lipoprotein complexes. The capillary electrophoresis system separates complex mixtures by electrokinetic forc

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,Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles?(OMVs)e factors that can often induce complex pathologies far from the infection sites. The OMVs of ., often regarded as the . of BMVs are known to traffic a battery of specific small MW alkyl-quinolones (AQs). These AQs function like primordial hormones by modulating intra-species and inter-species bacte

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Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells,e transport/trafficking, endocytosis, cell cycle progression, cell contractility, and formation of stress fibers or focal adhesions. Functional activation of newly synthesized small monomeric GTPases is facilitated by a multi-step posttranslational process involving transferase-catalyzed addition of

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