Titlebook: High-Throughput Screening Assays in Toxicology; Hao Zhu,Menghang Xia Book 2016 Springer Science+Business Media New York 2016 Chemical toxi

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1064-3745 ology. serves as a valuable reference resource for translating new HTS techniques into standardized chemical toxicity assessment tools in order to advance modern toxicology research to a new era where HTS techniques can partially replace the prevailing animal models..978-1-4939-8171-7978-1-4939-6346-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Srilatha Sakamuru,Matias S. Attene-Ramos,Menghang Xia

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Using β-Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Si Using a quantitative high-throughput screening (qHTS) approach, we are able to screen large chemical libraries to profile potential small molecule modulators of the HIF-1 signaling pathway in a 1536-well format. This chapter describes two orthogonal cell based assays; one utilizing a β-lactamase re

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Better, Faster, Cheaper: Getting the Most Out of High-Throughput Screening with Zebrafishafish, with its well-documented advantages over many animal models, is now emerging as a true biosensor of chemical activity. Herein, we draw on nearly a decade of experience developing high-throughput toxicology screens in the developmental zebrafish to summarize the best practices in fulfilling th

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Using β-Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Sies or those with inadequate oxygen concentrations, activation of the HIF-1 transcription factor allows for subsequent activation of target gene expression implicated in cell survival. As a result, cells proliferate through formation of new blood vessels and expansion of vascular systems, providing n

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