Titlebook: Virus-Derived Nanoparticles for Advanced Technologies; Methods and Protocol Christina Wege,George P. Lomonossoff Book 2018 Springer Science

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CCMV-Based Enzymatic Nanoreactorsnt noncovalent methods are described to efficiently incorporate enzymes inside the capsid of these viral protein cages. The methods are based on pH, leucine zippers, and electrostatic interactions respectively, as a driving force for encapsulation. The methods are exclusively described for the enzym

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Book 2018tion of? VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems,? encapsulation of heterologous materials within VNPs, and the modification of the outer surface of VNPs and how such modified VNPs can be developed into functional entities. Written in the

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1064-3745 ation advice from the experts.This volume details protocols on virus-derived nanoparticles (VNPs) for a number of different applications. Chapters guide readers through the production of? VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems,? encapsula

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Fabrication of Tobacco Mosaic Virus-Like Nanorods for Peptide Displayions the TMV CP forms only disk-shaped macromolecules. Here, we describe the design, construction, purification, and processing of rod-shaped TMV-VLPs using a simple bacterial expression system. The robust nature of this system allows for the display of functional peptides and molecules on the outer surface of this novel VLP.

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Production and Application of Insect Virus-Based VLPsain strategies used to generate recombinant baculoviruses and the applications of insect virus-derived VLPs in basic and applied research. It then describes detailed protocols for generation of recombinant baculoviruses, screening for their expression of VLPs in insect cells, and VLP purification.

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Plant Virus-Based Nanoparticles for the?Delivery of Agronomic Compounds as a?Suspension Concentraterticle (PVN) when loaded with biologically active ingredients. Here we describe how to formulate a PVN loaded with the nematicide abamectin (Abm) beginning with the propagation of the virus through the formulation, deactivation, and characterization of the finished product.

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