Titlebook: Viral Applications of Green Fluorescent Protein; Methods and Protocol Barry W. Hicks Book 2009 The Editor(s) (if applicable) and The Author

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Adenovirus-Mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Termd by sequence-specific degradation of mRNA, which is triggered by short (19–28 nt) silencing RNAs (siRNA). Efficient gene silencing using RNAi has been demonstrated in numerous cell lines and primary cultured cells. Incorporation of siRNA into terminally differentiated mammalian cells, such as adult

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Production of Multicistronic HIV-1 Based Lentiviral Vectors,e transfer applications would benefit from vectors capable of expressing multiple genes. This chapter focuses on production of bicistronic and tricistronic lentiviral vectors based on the internal ribosomal entry site (IRES) sequence of encephalomyocarditis virus (EMCV) and/or foot-and-mouth disease

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