Titlebook: Vibrio Cholerae; Methods and Protocol Book 2018

HIKE

,, as a?Native Host Model for Understanding Pathophysiology of ,, a variety of aquatic animals, including vertebrate fish. Here we describe the use of zebrafish (.) as a model for the pathogenic life cycle of .. Being that fish are natural hosts for ., the model provides several significant advantages over existing mammalian models that are not natural hosts.

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Gyrate

Ondines-curse

Proteomics of ,,in, and obtained peptides are labeled with iTRAQ reagent and separated by strong cation exchange to reduce the complexity. Finally, the peptides are separated by reverse-phase chromatography, spotted on a MALDI target plate, and analyzed by MALDI TOF-TOF.

Mumble

pericardium

Genotypic and Phenotypic Assays to Distinguish , Biotype,o main biotypes that make up the . O1 serogroup, which each display unique genotypic and phenotypic characteristics that allow for reliable biotype characterization. While treatment for cholera is much the same despite the causative strain’s biotype, such classification can be imperative for laborat

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hardheaded

Random Transposon Mutagenesis of ,,nts that contribute to specific phenotypes. One such approach that has been used in . for many years is the introduction of mariner transposons to generate random libraries of mutants. These libraries have been successfully used for a wide variety of genetic screens and selections in this important

BARB

Natural Cotransformation and Multiplex Genome Editing by Natural Transformation (MuGENT) of ,, foreign DNA, is shared by diverse microbial species and can be exploited for making mutant strains. Canonically, this process has been used to generate single mutants and sequentially to generate strains with multiple mutations. Recently, we have described a method for multiplex genome editing by n

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