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Titlebook: Vertebrate Embryogenesis; Embryological, Cellu Francisco J. Pelegri Book 2019Latest edition Springer Science+Business Media, LLC, part of S

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發(fā)表于 2025-3-21 17:22:00 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Vertebrate Embryogenesis
副標題Embryological, Cellu
編輯Francisco J. Pelegri
視頻videohttp://file.papertrans.cn/983/982302/982302.mp4
概述Includes cutting-edge, fully updated techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Vertebrate Embryogenesis; Embryological, Cellu Francisco J. Pelegri Book 2019Latest edition Springer Science+Business Media, LLC, part of S
描述This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and fully updated, .Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition. serves as an invaluable aid to scientists, educators, and the advanced general audience a
出版日期Book 2019Latest edition
關(guān)鍵詞Xenopus; Zebrafish; Balbiani body; Mouse embryos; CRISPR-Cas9; Model systems; Non-traditional species
版次2
doihttps://doi.org/10.1007/978-1-4939-9009-2
isbn_ebook978-1-4939-9009-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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Methods in Molecular Biologyhttp://image.papertrans.cn/v/image/982302.jpg
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https://doi.org/10.1007/978-1-4939-9009-2Xenopus; Zebrafish; Balbiani body; Mouse embryos; CRISPR-Cas9; Model systems; Non-traditional species
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Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections,RNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.
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Generation of , Haploid, Triploid, and Hybrid Embryos,genetic relationships. Here we describe methods to generate haploid, triploid, and hybrid species starting from eggs and sperm of . and . that enable investigation of how genome size and content affect physiology at the organismal, cellular, and subcellular levels.
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Vertebrate Embryogenesis978-1-4939-9009-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
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