Titlebook: Vascular Biology Protocols; Nair Sreejayan,Jun Ren Book 2007 Humana Press 2007 cardiovascular.endothelium.hypertension.migration.smooth mu

肉體

Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells,e resulting in their migration, proliferation, and deposition of extracellular matrix within the neointimal tissue. This chapter describes a method of isolation of SMCs from rat aorta and in vitro assays to characterize these abnormal SMC functions.

BRINK

Book 2007scular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects..

箴言

Immunostaining of Mouse Atherosclerotic Lesions,llenges to ensuring that development of the chromogen is due to specific antigen–antibody interaction. Thus, the determination of specific interactions frequently requires the judicious use of appropriate control experiments.

Vital-Signs

Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice,se-deficient 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (Mykd-PFK-2) show reduced glucose transport and reduced glycolysis when compared with control. The metabolites glucose-6-phosphate, fructose-6-phosphate, and glycogen were elevated, whereas fructose-1,6-bisphosphate was reduced in the transgenic Mykd-PFK-2 mouse hearts.

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一加就噴出

Mechanical Measurement of Contractile Function of Isolated Ventricular Myocytes,t of certain “tricks” that have proven useful in enhancing the yield of viable cells from the whole heart. Mastering the isolation of myocytes will provide a much better framework for real-time beat-to-beat recording of myocyte contraction as well as intracellular Ca. transients.

deforestation

Rat Carotid Artery Balloon Injury Model,ury across species and in multiple vascular beds in a cumulative effort to relate these findings to the human condition. In this context, the rat carotid artery balloon injury model is highly characterized and commonly used for investigating gross morphological, cellular, biochemical, and molecular

PRE

Histological and Morphometric Analyses for Rat Carotid Balloon Injury Model,ed carotid artery balloon injury model in laboratory rats. This approach utilizes luminal insertion of a balloon embolectomy catheter into the common carotid artery with inflation and withdrawal resulting in an injury characterized by vascular endothelial cell (EC) denudation and medial wall distens

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Plaque Rupture Model in Mice,olved in the plaque rupture remain unknown. Pathological animal models are valuable in the research on human disease mechanism, their therapy, and drug development. Recently, we have proposed a novel murine model of atherosclerotic plaque rupture associated with not only intraplaque hemorrhage but a