Titlebook: Vaccinia Virus and Poxvirology; Methods and Protocol Stuart N. Isaacs Book 20041st edition Humana Press 2004

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Gentry

Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses,lating T7 RNA polymerase expression, and integration of .I under vaccinia promoter control into the viral genome, vT7lacOI, yielded a recombinant virus capable of IPTG-inducible T7 promoter-controlled expression of foreign genes.

VEIL

Working Safely with Vaccinia Virus,roblems in normal hosts. This chapter reviews standard operating procedures when working with vaccinia virus and issues surrounding the use of prophylactic smallpox vaccination for laboratory workers.

Mutter

Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers,detail two different selection procedures: one relies on plaque formation phenotype using the vaccinia virus gene F13L; the other relies on antibiotic resistance using the . xanthine-guanine phosphoribosyl transferase gene.

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Orthopoxvirus Diagnostics,h three different methods that should be suitable for the preparation of orthopoxvirus DNA from various sources (e.g., clinical specimens or cell cultures) and four different methods for PCR that should be useful for investigating orthopoxvirus species and strains.

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蝕刻

Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface Plasmon Resonance,s basic procedures for employing SPR to study the interaction between poxvirus proteins and host immune-signaling proteins. We also identify how this methodology may be adapted toward other applications relevant in the study of poxvirus-host interactions.

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Working Safely with Vaccinia Virus,interactions, and as a protein expression system and a vaccine vector. The ubiquitous use of vaccinia viruses in the laboratory raises certain safety concerns, because the virus can be a pathogen in individuals with immunological and dermatological abnormalities and, on occasion, can cause serious p