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Titlebook: Vaccinia Virus and Poxvirology; Methods and Protocol Stuart N. Isaacs Book 2012Latest edition Springer Science+Business Media, LLC 2012 Vac

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書目名稱Vaccinia Virus and Poxvirology
副標(biāo)題Methods and Protocol
編輯Stuart N. Isaacs
視頻videohttp://file.papertrans.cn/981/980213/980213.mp4
概述Details the most current poxvirus protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Vaccinia Virus and Poxvirology; Methods and Protocol Stuart N. Isaacs Book 2012Latest edition Springer Science+Business Media, LLC 2012 Vac
描述.Since the first edition of .Vaccinia Virus and Poxvirology: Methods and Protocols. was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.S. military and relatively large numbers of U.S. civilians.?Novel anti-poxvirus therapeutics have been developed and have been used in emergency settings. The second edition of .Vaccinia Virus and Poxvirology. expands upon the previous edition with entirely new sets of protocols. Written in the highly successful .Methods in Molecular Biology?. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls..?Authoritative and practical,. Vaccinia Virus and Poxvirology: Methods and Protocols,. Second Edition. seeks to aid scientists in continuing to study poxviruses using new tools and approaches..
出版日期Book 2012Latest edition
關(guān)鍵詞Vaccinia virus; in vivo models; infectious disease; poxvirology; poxvirus bioinformatics; poxvirus immuno
版次2
doihttps://doi.org/10.1007/978-1-61779-876-4
isbn_softcover978-1-4939-6083-5
isbn_ebook978-1-61779-876-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2012
The information of publication is updating

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Easy and Efficient Protocols for Working with Recombinant Vaccinia Virus MVA, expression of recombinant genes in the research laboratory and in biomedicine for vaccine development. Major benefits of MVA include the clear safety advantage compared to conventional vaccinia viruses, the longstanding experience in the genetic engineering of the virus, and the availability of est
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Isolation of Recombinant MVA Using F13L Selection,MVA technology, the isolation of recombinant viruses remains a tedious and difficult process. This chapter describes the use of an efficient and easy-to-use selection system adapted for MVA. The system is based on the requirement of the viral gene F13L for efficient virus spread in cell culture, whi
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Imaging of Vaccinia Virus Entry into HeLa Cells,monitor virus motility, viruses or cellular proteins are fused with fluorescence markers and then detected by time-lapse fluorescence microscopy. These techniques allow kinetic analyses of individual virus particles in motion during the virus entry process as well as monitoring of dynamic interactio
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Measurements of Vaccinia Virus Dissemination Using Whole Body Imaging: Approaches for Predicting of such as Western Reserve (WR) strain or other orthopoxviruses. Traditionally, efficacy of treatment is evaluated using various readouts, such as lethality (rare), measurements of body weight loss, pox lesion scoring, and determination of viral loads in internal organs by enumerating plaques in sensi
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