Titlebook: Vaccine Design; Methods and Protocol Sunil Thomas Book 2016 Springer Science+Business Media New York 2016 Veterinary disease.Farm animals.A

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Development of Fasciola Vaccine in an Animal Modelll as humans in the future. The purpose of this chapter is to provide a protocol to develop a recombinant protein-based vaccine against . infection in mice. Moreover, this method can also be used as a guideline when the vaccination is performed in larger animals.

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DNA Vaccination in Chickensalso be administered in ovo, and thus, mass vaccination and early induction of immune response can effectively be achieved. In this chapter, we focus on the development of DNA vaccines against important infectious viral as well as parasitic diseases of poultry.

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Prokaryotic Production of Virus-Like Particle Vaccine of Betanodavirusan be expressed in vitro either by Baculovirus-based or yeast-based eukaryotic system or by bacterial expression system. In this chapter, the prokaryotic expression and the subsequent purification of VLP of betanodavirus orange-spotted grouper nervous necrosis virus (OGNNV) are presented.

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Design and Construction of Shrimp Antiviral DNA Vaccines Expressing Long and Short Hairpins for Protomising new approach to vaccination. Plasmid DNA vaccines that express short or long double stranded RNA in vivo have shown protection against viral diseases. The design, construction and considerations for preparing such vaccines are discussed.

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