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Titlebook: VEGF Signaling; Methods and Protocol Lorna R. Fiedler,Caroline Pellet-Many Book 2022Latest edition The Editor(s) (if applicable) and The Au

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發(fā)表于 2025-3-21 18:12:02 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱VEGF Signaling
副標(biāo)題Methods and Protocol
編輯Lorna R. Fiedler,Caroline Pellet-Many
視頻videohttp://file.papertrans.cn/981/980043/980043.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: VEGF Signaling; Methods and Protocol Lorna R. Fiedler,Caroline Pellet-Many Book 2022Latest edition The Editor(s) (if applicable) and The Au
描述This volume provides an updated collection of protocols for manipulating and studying VEGF signaling pathways .in vitro. and .in vivo. and aims to present a range of both firmly established and newly emerging technologies. Covering multiple model species, from mouse to zebrafish to human, the book explores the role of VEGF and VEGFR isoforms in exosomes, cultured cells, or in tissues, as well as robust cell assays for the investigation of basic angiogenic mechanisms and VEGF signaling in more complex cellular systems, amongst other subjects. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and up-to-date, .VEGF Signaling: Methods and Protocols, Second Edition. provides a useful tool for researchers in the vascular biology community and beyond in understanding the basic biology of VEGF signaling and in translating this research into the clinic..
出版日期Book 2022Latest edition
關(guān)鍵詞Vascular Endothelial Growth Factor; VEGF and VEGFR isoforms; Vascular biology; Signaling pathways; Cardi
版次2
doihttps://doi.org/10.1007/978-1-0716-2217-9
isbn_softcover978-1-0716-2219-3
isbn_ebook978-1-0716-2217-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

書目名稱VEGF Signaling影響因子(影響力)




書目名稱VEGF Signaling影響因子(影響力)學(xué)科排名




書目名稱VEGF Signaling網(wǎng)絡(luò)公開(kāi)度




書目名稱VEGF Signaling網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




書目名稱VEGF Signaling被引頻次




書目名稱VEGF Signaling被引頻次學(xué)科排名




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Identification of VEGF Isoforms in Mouse, Rat, and Zebrafish Using RT-qPCR,ion of transcripts that may be present in low abundance. The technique also permits detection of mRNAs for different isoforms of proteins due to the exquisite specificity of carefully designed primers. Here I describe the detection of mRNAs for VEGF isoforms in mouse, rat and zebrafish. The protocol
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Absolute Quantification of Plasma Membrane Receptors Via Quantitative Flow Cytometry,hormones, and cytokines). The abundance of plasma membrane receptors can be a diagnostic or prognostic biomarker in many human diseases. One of the best techniques for measuring plasma membrane receptors is quantitative flow cytometry (qFlow). qFlow employs fluorophore-conjugated antibodies against
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Co-immunoprecipitation Assays,n one protein, you will also obtain any other proteins that exist in a complex with that protein. It is a relatively simple technique that does not require expensive reagents or materials. It is however, not without its limitations and some of these will be discussed here along with a step-by-step g
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Using Immortalized Endothelial Cells to Study the Roles of Adhesion Molecules in VEGF-Induced Signa or knockout technologies. However, many in vitro protocols, particularly those of a biochemical nature, require large numbers of endothelial cells. These types of analyses are encumbered by the need to repeatedly produce and characterize primary endothelial cell cultures and can be greatly facilita
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RNAscope for VEGF-A Detection in Human Tumor Bioptic Specimens, factors, and poor prognosis in many tumors. VEGF-A binds its receptor?2 (VEGFR2) to induce neo-angiogenesis, a constant hallmark of tumor initiation and progression. Based on VEGF-A/VEGFR2 relevance in tumor angiogenesis, several inhibitors were developed. However, the clinical benefits of anti-ang
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