| 書目名稱 | Type 3 Secretion Systems |
| 副標(biāo)題 | Methods and Protocol |
| 編輯 | Matthew L. Nilles,Danielle L. Jessen Condry |
| 視頻video | http://file.papertrans.cn/932/931360/931360.mp4 |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia |
| 叢書名稱 | Methods in Molecular Biology |
| 圖書封面 |  |
| 描述 | .This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful?.Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. Practical and comprehensive,?.Type 3 Secretion Systems: Methods and Protocols.?is a valuable resource for anyone int |
| 出版日期 | Book 2017 |
| 關(guān)鍵詞 | site-directed mutagenesis; interactions of T3S components; heterologous proteins; effector translocatio |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-4939-6649-3 |
| isbn_softcover | 978-1-4939-8260-8 |
| isbn_ebook | 978-1-4939-6649-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media New York 2017 |
|Archiver|手機(jī)版|小黑屋|
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