Titlebook: DNA Technology; Joseph R. Lakowicz Book 2003 Springer Science+Business Media New York 2003 Biosensor.biology.electrophoresis.fluorescence.

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Fluorescence in Nucleic Acid Hybridization Assays,sitivity, with time-resolved lanthanide fluorescence providing detection down to 0.01 amol, or about 6000 molecules of target nucleic acid. With amplification, the detection limit drops to the tens or hundreds of molecules, or even the single molecule level. For many applications amplification would

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Energy Transfer Fluorescent Labels for DNA Sequencing and Analysis,sion intensities and improved spectral purity provided by ET primers and terminators have led to a major improvement in the performance of automated DNA sequencers. Fluorescent ET dye-labels should be equally valuable for the analysis of PCR fragments, restriction fragments, single nucleotide polymo

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On-the-Fly Fluorescence Lifetime Detection in Capillary Electrophoresis for DNA Analysis,ined advantages of speed, high resolution, and small sample volume provided by CE and the accuracy and signal resolution provided by fluorescence lifetime in no more time than is needed for fluorescence intensity detection alone make frequency-domain lifetime detection a promising approach to proble

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Book 2003biquitous in molecular biology, medical diagnostics, and forensics. Much of the readout technology is based on fluorescence detection. This volume contains contributions from many experts in the field who present an overview of many aspects of DNA technology. These chapters provide an understanding

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Lanthanide-Labeled DNA,topic, avoid the many practical problems associated with radioactive probes. Lanthanide probes are particularly advantageous in resonance energy transfer, whether for measuring distances in biocomplexes or when used to generate new lifetime tailored dyes.

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