| 書目名稱 | Synthetic Messenger RNA and Cell Metabolism Modulation | | 副標題 | Methods and Protocol | | 編輯 | Peter M. Rabinovich | | 視頻video | http://file.papertrans.cn/885/884364/884364.mp4 | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | .Synthetic mRNA is an attractive tool for mammalian cell reprogramming that? can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing? the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. .Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols. covers the typical main methods, such as mRNA synthesis, modifications, and delivery.? Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful .Methods in Molecular Biology?. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls..?.Authoritative and easily accessible, | | 出版日期 | Book 2013 | | 關(guān)鍵詞 | cell metabolism; cell reprogramming; immunotherapy; mRNA synthesis; synthetic messenger RNA; transfection | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-62703-260-5 | | isbn_softcover | 978-1-4939-5967-9 | | isbn_ebook | 978-1-62703-260-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2013 |
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