Titlebook: Synthetic DNA; Methods and Protocol Randall A. Hughes Book 2017 Springer Science+Business Media, LLC, part of Springer Nature 2017 syntheti

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Synthetic Gene Design Using Codon Optimization On-Line (COOL)y developed Codon Optimization On-Line (COOL) which is a web-based tool to provide multi-objective codon optimization functionality for synthetic gene design. COOL provides a simple and flexible interface for customizing codon optimization based on several design parameters such as individual codon

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Simple Cloning by Prolonged Overlap Extension-PCR with Application to the Preparation of Large-Size me, ligase, exonuclease, or recombinase in .. This technology can be applied to common . hosts (e.g., DH5α, JM109, TOP10, BL21(DE3)). The protocol includes three steps: (1) generate DNA insert and linear vector backbone by regular high-fidelity PCR, where these two DNA fragments contain 3′ and 5′ ov

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BASIC: A Simple and Accurate Modular DNA Assembly Method assembly and provides highly accurate DNA assembly with 99 % correct assemblies for four parts and 90 % correct assemblies for seven parts [1]. The BASIC standard defines a single entry vector for all parts flanked by the same prefix and suffix sequences and its idempotent nature means that the ass

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Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotidesnostics, nanotechnology, and gene therapy. They are usually chemically synthesized. Here we describe an enzymatic method which enables us to synthesize pure oligonucleotides which can be up to several hundred long bases.

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The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesisom multiple off-products. This amplification step can cause stochastic sampling effects that propagate errors during the synthesis and lower the variability when applied towards the construction of randomized libraries. We present the method for polymerase step reaction (PSR), a simple DNA polymeras

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