Titlebook: Somatic Stem Cells; Methods and Protocol Shree Ram Singh,Pranela Rameshwar Book 2018Latest edition Springer Science+Business Media, LLC, pa

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Methods of Mesenchymal Stem Cell Homing to the Blood–Brain Barriery by differentiating into specialized cells of all germ layers. MSCs are of relevant to medicine and have been proposed for several disorders. MSCs can be transplanted across allogeneic barriers as “off the shelf” cells. This chapter focuses on methods to deliver MSCs to the brain because neurologic

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3D Bioprinting and Stem Cellse complex endogenous conditions in the body. Additionally, fabrication technology is more accessible than ever. Bioprinting, in particular, is an additive manufacturing technique that expands the capabilities of in vitro studies by precisely depositing cells embedded within a 3D biomaterial scaffold

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Detection, Labeling, and Culture of Lung Stem and Progenitor Cellscellular and molecular mechanisms that regulate their fate. Akin to development in vivo, the in vitro growth of adult lung epithelial stem cells requires support of mesenchymal-derived growth factors. In the adult mouse lung, epithelial stem/progenitor cells are defined by the phenotype CD45neg CD31

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Isolating and Characterizing Adipose-Derived Stem Cellsle and rich source of adult stem cells, termed adipose-derived stem cells (ASCs). ASCs have the most important advantage over stem cells from other available sources. There is no other human tissue as abundant as adipose tissue, making it possible to isolate adequate numbers of ASCs for potential cl

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Identification and Characterizations of Annulus Fibrosus-Derived Stem Cellsever, repair or regeneration of AF remains challenging. Recently, we have found that there exists a subpopulation of cells, which form colonies in vitro and could self-renew, in AF tissue. These cells express typical surface antigen molecules of mesenchymal stem cells, including CD29, CD44, and CD16