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Titlebook: Shotgun Proteomics; Methods and Protocol Mónica Carrera,Jesús Mateos Book 2021 Springer Science+Business Media, LLC, part of Springer Natur

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書目名稱Shotgun Proteomics
副標題Methods and Protocol
編輯Mónica Carrera,Jesús Mateos
視頻videohttp://file.papertrans.cn/867/866759/866759.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Shotgun Proteomics; Methods and Protocol Mónica Carrera,Jesús Mateos Book 2021 Springer Science+Business Media, LLC, part of Springer Natur
描述.This edition details a collection of specific shotgun proteomics-based laboratory techniques and applications developed in leading laboratories and proteomics units worldwide. Chapters cover a broad range of topics covering, shotgun proteomics of extracellular vesicles and subcellular structures, shotgun proteomics in non-model organisms, clinical proteomics, food proteomics, analysis of post-translational modifications and protein complexes, and data processing and storage. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...?..Authoritative and cutting-edge,?.Shotgun Proteomics: Methods and Protocols .aims to be an up-to-date guide for researchers seeking to understand the proteome of any given biological sample..
出版日期Book 2021
關鍵詞hyperglycaemia; mass spectrometry; Label-Free quantification; Jurkat T-cells; carbamylation; Proteomics
版次1
doihttps://doi.org/10.1007/978-1-0716-1178-4
isbn_softcover978-1-0716-1180-7
isbn_ebook978-1-0716-1178-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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Clinical Proteomics for the Analysis of Circulating Extracellular Vesiclessed proteomics and bottom-up and top-down mass spectrometry have been applied to the study of EVs. In this chapter, we will present basic protocols for gel-based and quantitative MS-based approaches applied to the study of EVs.
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Serum Proteomic Profiling in Rheumatoid Arthritis by Antibody Suspension Bead ArraysRF, in particular RF-IgM, has the great potential to interfere with antibody-based immunoassays by nonspecifically binding capture antibodies. Because of this concern, we describe a procedure to reduce the interference of RF-IgM on RA serum protein profiling approaches based on multiplexed antibody suspension bead arrays.
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Clinical Proteomics for the Analysis of Circulating Extracellular Vesiclesthe study of disease progression and drug resistance. The clinical potential of circulating extracellular vesicles (EVs) as a source of biomarkers is one of the reasons why several research groups have recently applied proteomics to their study. A large variety of proteomic approaches such as gel-ba
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Utilization of Laser Capture Microdissection Coupled to Mass Spectrometry to Uncover the Proteome ofr structures, for further proteomic study. Furthermore, mass spectrometry (MS) can quickly and accurately generate differential protein expression profiles from small amounts of samples. Although cellular protrusions—such as tunneling nanotubes, filopodia, growth cones, invadopodia, etc.—are involve
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Isolation of Apoplastic Fluid from Woody Plant Leaves: Grapevine and Coffee as a Case Studytion. Technical advances allowed a deeper characterization of plant proteomes, highlighting the need to study cellular compartments. The apoplast is the cellular compartment external to the plasma membrane including the cell wall, where a broad range of processes take place including intercellular s
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