Titlebook: Shiga Toxin-Producing E. coli; Methods and Protocol Stephanie Schüller,Martina Bielaszewska Book 2021 Springer Science+Business Media, LLC,

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Integrated Approach for the Diagnosis of Shiga Toxin-Producing , Infections in Humans,diagnosis of STEC infection is of primary importance to drive the most appropriate patient’s management procedures. The methods to diagnose STEC infections include both direct isolation of the STEC from stool samples and the identification of indirect evidences based on molecular, phenotypic, and se

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Shiga Toxin-Producing , in Animals: Detection, Characterization, and Virulence Assessment,ic potential for humans. Domestic swine can carry and shed STEC, but only STEC strains producing the Shiga toxin (Stx) 2e variant and causing edema disease in piglets are considered pathogens of veterinary medical interest. In this chapter, we present general diagnostic workflows for sampling livest

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Identification of Shiga Toxin-Producing , Outbreaks Using Whole Genome Sequencing,iate them from sporadic cases. Here, we describe an optimized protocol to efficiently determine the genome sequences of STEC using short read Illumina technology and provide information on helpful tools for the subsequent bioinformatic analysis.

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Predicting Host Association for Shiga Toxin-Producing , Serogroups by Machine Learning,w considerable diversity in gene content between isolates. It is a reasonable assumption that gene content reflects evolution of strains in particular host environments and therefore can be used to predict the host most likely to be the source of an isolate. An extrapolation of this argument is that

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Isolation and Characterization of Shiga Toxin Bacteriophages,e methods used for the detection, enumeration, and isolation of Stx bacteriophages. Stx phages are temperate phages located in the genome of STEC. Their induction from the host strain cultures is achieved by different inducing agents, mitomycin C being one of the most commonly used. Detection of inf

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Functional Analysis of Shiga Toxin-Producing , Biofilm Components in Plant Leaves,orticultural crops. This becomes a public health concern for plants that are eaten raw or minimally processed, such as leafy salad and fruits. STEC actively interact with plants as hosts, and so to determine the mechanistic basis to the interaction, it is necessary to assess STEC gene function in pl

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Virulence Factor Cargo and Host Cell Interactions of Shiga Toxin-Producing , Outer Membrane Vesiclehese pathogens. STEC O157 OMVs carry a cocktail of virulence factors including Shiga toxin 2a (Stx2a), cytolethal distending toxin V (CdtV), EHEC hemolysin, flagellin, and lipopolysaccharide. OMVs are taken up by human intestinal epithelial and microvascular endothelial cells, the major targets duri

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