Titlebook: Salamanders in Regeneration Research; Methods and Protocol Anoop Kumar,András Simon Book 2015 Springer Science+Business Media New York 2015

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In Vivo Modulation and Quantification of microRNAs During Axolotl Tail Regenerationledge of the molecular mechanisms that regulate this amazing ability is still in its infancy. Many recent papers have shown important roles for microRNAs in regulating regeneration in a number of species. The ability to detect and quantify miRNA expression fluctuations at a single cell level in vivo

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Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemasmander) limb, dissection of molecular mechanisms, as in other species, can be greatly aided by robust in vitro models. This chapter focuses on derivation and maintenance of cell lines from adult post-metamorphic salamanders and in particular cells derived from normal and regenerating limbs. We also

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Culture and Transfection of Axolotl Cellsbility to grow cells from multicellular organisms like humans, squids, or salamanders is important to simplify the analyses and experimental designs to help understand the biology of these organisms. The advent of the first cell culture has allowed scientists to tease apart the cellular functions, a

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Book 2015rs in Regeneration Research: Methods and Protocols .guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on targeting a wide variety of structures, ranging from the limb to the heart and to the brain, and methods for studying genetically modified organis

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Housing and Maintenance of ,, the Mexican Axolotltively straightforward maintenance and their versatility as an animal model for development and regeneration. Interest in using the axolotl in laboratory research has grown tremendously over the past decade, so dedicated resources to support the study of this species are needed and encouraged.

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1064-3745 ation advice from the experts.Includes supplementary materia.This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. .Salamanders in Regeneration Research: Methods and Protocols .guides readers through experimental ma