Titlebook: SUMO; Methods and Protocol Manuel S. Rodriguez Book 2016 Springer Science+Business Media New York 2016 small ubiquitin-related modifier SUM

AMBI

ensemble

招惹

心神不寧

The Use of Multimeric Protein Scaffolds for Identifying Multi-SUMO Binding Proteinsasily expressed and purified from bacterial cells for use as baits in pull-down assays. This methodology facilitates the discovery of novel SUMO-binding proteins or further characterization of SUMO with known binding partners.

Flagging

Isolation of In Vivo SUMOylated Chromatin-Bound Proteinsrecipitates chromatin-associated fractions of SUMOylated heterochromatin protein 1α in cultured cells. Techniques to enrich endogenous SUMO substrates at the chromatin are also demonstrated and discussed. This approach could be adapted to evaluate chromatin-bound SUMO targets in additional in vivo systems.

Blatant

Concepts and Methodologies to Study Protein SUMOylation: An Overviewgh distinct names were proposed including Sentrin, GMP1, PIC1, or SMT3, SUMO became the most popular. Early studies on the functions of SUMOylation focused on activities in the nucleus, including transcription activation, chromatin structure, and DNA repair. However, it is now recognized that SUMOyl

plasma

The Regulation of Chromatin by Dynamic SUMO Modificationscal role for SUMO in modulating gene expression. Dynamic sumoylation of transcription factors, chromatin-modifying enzymes, histones, and other chromatin-associated factors significantly affects the transcriptional status of the eukaryotic genome. Recent studies have employed high-throughput ChIP-Se