Titlebook: SILAC; Methods and Protocol Jose L. Luque-Garcia Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

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Heavy Methyl SILAC Metabolic Labeling of Human Cell Lines for High-Confidence Identification of R/Kh-confidence identification of in vivo methyl-peptides by MS-based proteomics. We provide a general protocol that covers the steps of heavy methyl labeling of cultured cells, protein sample preparation, LC-MS/MS analysis, and downstream computational analysis of the acquired MS data.

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SILAC-Based Quantitative Phosphoproteomics in Yeast,e..The combination of SILAC-based quantitation with phosphopeptides enrichment by TiO. in a batch that enables measurement of protein posttranslational modifications is a powerful application to analyze the global phosphoproteome for studies in signaling pathways.

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天空

SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos,cy of over 92%. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.

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Use of Nuclear and Chromatin Enrichment Procedures for Quantitation of Yeast DNA Replication Protei)-based quantitative proteomics. Here I describe a detailed methodology for SILAC labeling of budding yeast ., then nuclear isolation and chromatin preparation from synchronized yeast cells, prior to quantitative proteomic analysis of DNA replication proteins.

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Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trappine these classes of proteins in affinity-purified mixtures. Here we describe the use of stable isotope labeling by amino acids in cell culture, substrate trapping, and mass spectrometry to enable the objective identification of the components of affinity-purified protein complexes.

外向者

Dynamic SILAC to Determine Protein Turnover in Neurons and Glia,fferent rates depending on various factors including cell type, subcellular localization, cellular environment, and neuronal activity. Here we describe a workflow for the analysis of protein synthesis, degradation, and turnover in primary cultured rat neurons and glia using dynamic/pulsed SILAC and mass spectrometry.

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暫時中止

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