Titlebook: Rotaviruses; Methods and Protocol James Gray,Ulrich Desselberger Book 2000 Humana Press 2000

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Immunologic Methods and Correlates of Protection,une responses has included study of the importance of circulating vs intestinal antibodies (Abs), serotype-specific vs group-specific Abs, and RV-specific immunoglobulins IgA, IgM, and IgG (.). Following natural RV infection, RV-specific IgM, followed by IgA and IgG, appear in serum and duodenal flu

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In Vivo Study of Immunity to Rotaviruses,ance of primary RV infection, and the mechanisms that mediate immunity to reinfection. The use of immunodeficient strains of mice, immunodepletion studies with specific monoclonal antibodies (MAbs), and passive transfer of purified cells are three basic, complementary experimental approaches that ha

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Methods of Rotavirus Detection, Sero- and Genotyping, Sequencing, and Phylogenetic Analysis, causes of gastroenteritis. Therefore, laboratory procedures, including electron microscopy (EM) (.), enzyme-linked immunosorbent assays (ELISA) (.), passive particle agglutination tests (PPAT) (.,.), polyacrylamide gel electrophoresis (PAGE) (.), or reverse transcription-polymerase chain reaction (

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Epidemiology of Group A Rotaviruses Surveillance and Burden of Disease Studies,re gastroenteritis (GE). Each year, Group A RVs are estimated to cause approx 870,000 deaths worldwide in children less than 5 years (yr) of age, mostly in developing countries (.). This chapter will describe the epidemiological features of Group A RV infections, and will critically review the curre

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Future Rotavirus Research, structure, classification, evolution, replication, pathogenesis, and specific immune responses, and their correlation with protection and epidemiology. As pathogens that infect the gastrointestinal tract, these viruses continue to serve as useful models to understand mucosal virus-cell interactions

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Book 2000 structure, molecular biology, pathogenesis, epidemiology, and prevention in animal models of infection with rotaviruses, an important cause of infant morbidity and mortality. Presented by experts in the fields of animal and human rotavirus infections and rotavirus vaccine research, these readily re

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In Vivo Study of Immunity to Rotaviruses,ional in immunocompetent mice. Immunodepletion experiments introduce into the experimental animal high (nonphysiological) levels of antibodies (Abs) that potentially have other immunomodulatory effects different from the desired one, and, many times, depletion strategies fail to completely eliminate