Titlebook: Rice Genome Engineering and Gene Editing; Methods and Protocol Anindya Bandyopadhyay,Roger Thilmony Book 2021 Springer Science+Business Med

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Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice adopted as two of the most powerful genome editing systems in plants. Recently, we developed a single transcript unit (STU) CRISPR 2.0 toolbox for applications in plants, which contains two STU-Cas9 systems and one STU-Cas12a system. Here, we describe a detailed protocol about using the STU CRISPR

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Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9e effector nuclease (TALEN), such as multiplex genome editing. With multiplex genome editing, CRISPR-Cas9 becomes a preferred SSN to edit many quantitative trait loci (QTL) simultaneously for trait improvement in major crops. A multiplexed CRISPR system is also important for deletion of a large frag

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FLP-Mediated Site-Specific Gene Integration in Rice is by using the yeast FLP-. recombination system that is efficient in directing DNA integration into the “engineered” genomic sites. The critical parameters of this method include the use of the thermostable version of FLP protein and the promoter trap design to select site-specific integration clo

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Morphogenic Regulators and Their Application in Improving Plant Transformation elite germplasm of major crop species. This chapter will briefly review key regulatory genes involved in plant morphogenesis with a focus on in vitro somatic embryogenesis and their application in improving plant transformation.

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