Titlebook: Ribozymes; Methods and Protocol Robert J Scarborough,Anne Gatignol Book 2021 Springer Science+Business Media, LLC, part of Springer Nature

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書目名稱Ribozymes影響因子(影響力)




書目名稱Ribozymes影響因子(影響力)學(xué)科排名




書目名稱Ribozymes網(wǎng)絡(luò)公開度




書目名稱Ribozymes網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Ribozymes被引頻次




書目名稱Ribozymes被引頻次學(xué)科排名




書目名稱Ribozymes年度引用




書目名稱Ribozymes年度引用學(xué)科排名




書目名稱Ribozymes讀者反饋




書目名稱Ribozymes讀者反饋學(xué)科排名

MURAL

Cloning and Detection of Genomic Retrozymes and Their circRNA Intermediateser retrotransposons, transcription of retrozymes in vivo leads to the formation and accumulation of circular RNAs, which can be readily detected by RNA blotting. In this chapter, we describe the procedures needed to carry out the cloning of genomic retrozymes, and to detect by northern blot their circular RNA retrotransposition intermediates.

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Book 2021 on troubleshooting and avoiding known pitfalls...?Authoritative and cutting-edge, .Ribozymes: Methods and Protocols .aims to help in accelerating ribozyme research and inspiring others to develop new methods to study ribozyme structure and function..

ALERT

Conjugation as a Highly Sensitive Assay to Study Group II Intron Splicing In Vivomine the splicing competence of various Ll.LtrB mutants, whereas the splicing selection/conjugation assay was established to isolate splicing-proficient variants from a randomly generated bank of mutated introns.

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Co-transcriptional Analysis of Self-Cleaving Ribozymes and Their Ligand Dependenceication or additional preparation steps, such as refolding procedures. Furthermore, because the transcripts are not denatured, this assay likely yields RNAs in conformations relevant to co-transcriptionally folded species in vivo.

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