Titlebook: Ribozyme Protocols; Philip C. Turner Book 1997 Springer Science+Business Media New York 1997

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愚笨

BURSA

柔聲地說

A Practical Method for the Production of RNA and Ribozymes,des. Although great progress has been made in improving the methods for DNA synthesis, such that production of large amounts has become commonplace, similar quantities of RNA are still difficult to obtain.

objection

Preparation of Templates for Production of Ribozymes and Substrates, by in vitro transcription can be used as substrates in a variety of experiments ranging from splicing and translation to structural studies. This chapter describes methods for the preparation of templates suitable for in vitro transcription.

quiet-sleep

Enzymatic Synthesis and Characterization of Unmodified Ribozymes and Substrates,s can be end-labeled. Simple procedures generate a sequence ladder to confirm colinearity of template and transcript (.,.). This is not a “mere luxury,” since examples with significant template slippage have been reported (.). If a small labeled cleavage product is produced, also the exact cleavage sites can be determined (.).

overhaul

Minimized Hammerhead Ribozymes,form stem-loop II, and nucleotides at the 5′- and 3′-ends, which form helix I and helix III, respectively, in complex with the substrate. Such a ribozyme is shown in .. Most hammerhead ribozymes of this type are constructed with 12 nucleotides in stem-loop II, with four nucleotides in the loop and eight nucleotides forming a 4 bp double helix.

cancellous-bone

SPALL

永久

Qiao Yu,John M. Burkener Absenkung von Sedimenten kommt es unter zunehmenden geothermischen Einflu?, da normalerweise eine Temperaturzunahme von 3°C pro 100m Sedimentteufe (geothermischer Gradient) anzutreffen ist. Die Folge ist, da? sich das organische Material allm?hlich ver?ndert, indem nur thermisch stabile Produkte