Titlebook: Rheumatoid Arthritis; Methods and Protocol Shuang Liu Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclus

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Paraplegia

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Long-Term Constant Subcutaneous Drug Administrationic Pumps osmotic pump, continuous zero-order delivery of drugs under investigation that need repeated oral or intravenous dosing is realizable. Compared to traditional delivery systems, implanted osmotic pumps present several advantages, such as that no external connections or researcher interventio

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Clinical Scoring of Disease Activity in Animal Modelsstological examination of joints. Clinical scoring using a qualitative scoring system of paw inflammation (paw thickness, width, or volume) over time is the standard method used for subjective quantification of arthritis activity. To evaluate paw swelling status, a quantitative method using three-di

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Histological Analyses of Arthritic Joints in Collagen-Induced Arthritis Model Miceammatory disease characterized by increased synovial tissue and osteoclasts, angiogenesis, infiltration of inflammatory cells, and pannus formation. These pathologies can be observed in a collagen-induced arthritis model mouse using formaldehyde-fixated paraffin-embedded (FFPE) samples. For the prep

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Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis Systemrane proteins. However, developing antibodies against membrane proteins remains a big challenge because mass production of membrane proteins is difficult. Recently, we developed a highly efficient cell-free production method of proteoliposome antigen using a cell-free protein synthesis method with l

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Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen diseases. Analysis of autoantibodies is essential for investigating pathology mechanisms, diagnosis, and therapeutics of these diseases. We developed an autoantibody profiling assay using a cell-free synthesized protein array and high-throughput screening technology. Our assay system can sensitivel

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Generation of Specific Aptamersherapeutic molecules with high affinity and specificity and have been developed as potent drugs for the treatment of rheumatoid arthritis. Aptamers have unique and advantageous features over antibodies, such as superior affinity with nano- or pico-molar dissociation constants and ease of chemical sy