Titlebook: Reverse Genetics of RNA Viruses; Methods and Protocol Daniel R. Pérez Book 2024Latest edition The Editor(s) (if applicable) and The Author(

Conquest

Reverse Genetics of Dengue Virus,pulation of DENV genomes is a major molecular challenge, especially when using conventional cloning systems. To circumvent this issue, we describe a simple and efficient yeast-based reverse genetics system to recover infectious DENV clones.

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Reverse Genetics System for Rift Valley Fever Virus,ly from cDNAs containing predetermined mutations in their genomes to investigate virus–host interactions and mechanisms of pathogenesis and generate live-attenuated vaccines. In this chapter, we will describe the experimental procedures for the implementation of RVFV reverse genetics.

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Reverse Genetics Systems for Filoviruses, development of countermeasures, reverse genetic systems, and especially those facilitating the generation of recombinant filoviruses, are indispensable. Here, we describe the generation of recombinant filoviruses from cDNA.

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Reverse Genetics Systems for the De Novo Rescue of Diverse Members of ,rs circulate within diverse animal reservoirs. Concerningly, bat-borne paramyxoviruses have spilled over into humans with increasing frequency in recent years, resulting in severe disease. The risk of future zoonotic outbreaks, as well as the persistence of paramyxoviruses that currently circulate w

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