Titlebook: Retinoids; Methods and Protocol Hui Sun,Gabriel H. Travis Book 2010 Springer Science+Business Media, LLC 2010 Transport.Vitamin.Vivo.biolog

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Quantification of Endogenous Retinoids,ion, and all aspects of reproduction. Reliable retinoid quantification requires suitable handling and, in some cases, resolution of geometric isomers that have different biological activities. Here we describe procedures for reliable and accurate quantification of retinoids, including detailed descr

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Physiological Studies of the Interaction Between Opsin and Chromophore in Rod and Cone Visual Pigmee, 11-.retinal. Activation of the visual pigment by light triggers a transduction cascade that produces experimentally measurable electrical responses in photoreceptors. The interactions between opsin and chromophore can be investigated with electrophysiologial recordings in intact amphibian and mou

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Measurement of the Mobility of All-,-Retinol with Two-Photon Fluorescence Recovery After Photobleacow aqueous solubility, which makes the presence of carrier proteins and the spatial arrangement of cellular membranes especially relevant. In rod photoreceptor outer segments, all-.retinol is generated after light exposure from the reduction of all-.retinal that is released from bleached rhodopsin.

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,HPLC?/?MSN Analysis of Retinoids,key regulators of gene expression, retinol being oxidized via a retinaldehyde intermediate to retinoic acid (RA) which activates specific nuclear receptors, the signalling of which is turned off by oxidative inactivation of the ligand to 4-oxo-RA and other metabolites. Many of these retinoids are pr

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Binding of Retinoids to ABCA4, the Photoreceptor ABC Transporter Associated with Stargardt Macular es from rod and cone photoreceptor cells following photoexcitation as part of the visual cycle. Mutations in ABCA4 are known to cause Stargardt macular degeneration and related disorders, associated with a severe loss in vision. Recently, a solid-phase binding assay has been developed to identify re

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