Titlebook: Retinal Ganglion Cells; Methods and Protocol Ben Mead Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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Intravitreal Injection of AAV for the Transduction of Mouse Retinal Ganglion Cells,avitreal injection, which introduces the treatment into the largest cavity at the posterior of the eye. This technique is frequently used to deliver gene therapies, including those containing recombinant adeno-associated viral vectors (AAVs), to the back of the eye to enable inner retinal targeting.

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甜瓜

microscopic

Differentiation of Human Embryonic/Induced-Pluripotent Stem Cells to Retinal Ganglion Cells, a protocol for differentiation of hESC to RGCs using a 40-day protocol, significantly shorter than typical retinal organoids while still yielding cells with RGC-enriched markers and show physiological and morphological properties typical of RGCs.

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Optic Nerve Crush Injury in Rodents to Study Retinal Ganglion Cell Neuroprotection and Regeneration in accessing the optic nerve and creating a crush injury. This can be used to create a reproducible model to study the response of retinal ganglion cells (RGC), the main projection neurons of the eye, to injury.

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Optical Coherence Tomography: Imaging Visual System Structures in Mice,itor retinal layer thickness in healthy and diseased retinas in murine lines in vivo longitudinally. Thus, OCT can provide insights into disease severity and treatment efficacy. This chapter describes the use of OCT as a powerful non-invasive imaging technology for high-resolution retinal imaging and retinal thickness quantification in rodents.

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MOAT

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