Titlebook: Retinal Development; Methods and Protocol Chai-An Mao Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 central

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Adeno-Associated Virus as Gene Delivery Vehicle into the Retina,used by Leber congenital amaurosis or retinitis pigmentosa was approved by the US Food and Drug Administration in 2017. This is an exciting era for investigators working on retina biology and treatments for blindness. In this chapter, we provide detailed methods for laboratory-scale production, purification, and characterization of AAV.

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A Simple Guide for Generating BAC Transgenic Animals for Retinal Research,ze its endogenous regulation of expression using a reporter protein in vivo. Here, we illustrate the generation of a BAC:LIF-EGFP transgenic mouse line to describe a simplified version of BAC transgenesis using .-based recombineering.

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1064-3745 ation advice from the experts.This volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion cell morphology, gap-junction-mediated

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