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Titlebook: Resin Microscopy and On-Section Immunocytochemistry; Geoffrey R. Newman,Jan A. Hobot Book 2001Latest edition Springer-Verlag Berlin Heidel

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發(fā)表于 2025-3-21 16:25:19 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱Resin Microscopy and On-Section Immunocytochemistry
編輯Geoffrey R. Newman,Jan A. Hobot
視頻videohttp://file.papertrans.cn/829/828432/828432.mp4
概述Ready-to-go protocols.Explains all steps from fixation to labelling and detection.Helps in finding the resin of choice for your needs
叢書(shū)名稱Springer Lab Manuals
圖書(shū)封面Titlebook: Resin Microscopy and On-Section Immunocytochemistry;  Geoffrey R. Newman,Jan A. Hobot Book 2001Latest edition Springer-Verlag Berlin Heidel
描述Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This labmanual covers the embedding of tissue using epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities and results are discussed so that an understanding of the techniques can be acquired and appropriate choices made. The various resins available and all steps involved in tissue processing, beginning with fixation, as well as the great variety of labelling methods and markers that are commonly used for "on-section" cytochemistry and immunocytochemistry are described, including detailed protocols for the application.
出版日期Book 2001Latest edition
關(guān)鍵詞crytotechniques; electron microscopy; immunolabelling; resin embedding; tissue; tissue fixation
版次2
doihttps://doi.org/10.1007/978-3-642-56930-2
isbn_softcover978-3-642-47731-7
isbn_ebook978-3-642-56930-2
copyrightSpringer-Verlag Berlin Heidelberg 2001
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沙發(fā)
發(fā)表于 2025-3-21 22:52:10 | 只看該作者
Resin Microscopy and On-Section Immunocytochemistry978-3-642-56930-2
板凳
發(fā)表于 2025-3-22 03:07:16 | 只看該作者
The Strategic Approachserve the finest ultrastructural detail. Dehydration in an organic solvent gradient is then followed by embedding in an epoxy resin. Araldite for example, is as popular now as it was shortly after the first description of its use by Glauert et al. (1956).
地板
發(fā)表于 2025-3-22 04:53:51 | 只看該作者
as well as the great variety of labelling methods and markers that are commonly used for "on-section" cytochemistry and immunocytochemistry are described, including detailed protocols for the application.978-3-642-47731-7978-3-642-56930-2
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發(fā)表于 2025-3-22 11:22:25 | 只看該作者
Geoffrey R. Newman,Jan A. Hobot, and deduce linearization theorems. The results are illustrated using temporal and full discretizations of evolutionary differential equations.978-3-642-14257-4978-3-642-14258-1Series ISSN 0075-8434 Series E-ISSN 1617-9692
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, and deduce linearization theorems. The results are illustrated using temporal and full discretizations of evolutionary differential equations.978-3-642-14257-4978-3-642-14258-1Series ISSN 0075-8434 Series E-ISSN 1617-9692
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Geoffrey R. Newman,Jan A. Hobot, and deduce linearization theorems. The results are illustrated using temporal and full discretizations of evolutionary differential equations.978-3-642-14257-4978-3-642-14258-1Series ISSN 0075-8434 Series E-ISSN 1617-9692
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