Titlebook: Reshaping Urban Conservation; The Historic Urban L Ana Pereira Roders,Francesco Bandarin Book 2019 Springer Nature Singapore Pte Ltd. 2019

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cular level to increase yield as well as quality of important crops. This in turn has led to the development of techniques to induce beneficial variations in the genomic structure of plants. The success of such method relies on their flexibility and feasibility, which allows customization using avai

蒙太奇

binant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenes

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Francesco Bandarinsed mutational and DNA marker-based molecular breeding approaches have contributed enormously toward enhancement of rice productivity. However, it is becoming hard and hard to increase rice yield further due to several factors including a possible ceiling of rice yield potential, the frequent pest a

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Ana Pereira Roders to improve multiple traits but also for functional analysis of gene families. To solve the problem of delivery of many T-DNAs with separated binary vectors into plant cells at the same time, several approaches have been developed to express multiple gRNA cassettes from a single CRISPR/Cas9 vector.

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Amareswar Gallas) systems, transcription activator-like effector nucleases (TALENs), and zinc-finger nucleases (ZFNs)—and provides an assessment of the risk of misuse of these technologies based on the following parameters: accessibility, ease of misuse, magnitude of potential harm, and imminence of potential misu

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Loes Veldpaus,Helma Bokhovethe CRISPR/Cas system, the RNA-guided endonuclease Cas protein introduces a DNA double-stranded break at the genome position recognized by a guide RNA (gRNA) based on complementary base-pairing of about 20-nucleotides in length. The 8- or 12-mer gRNA sequence in the proximal region is especially imp