Titlebook: Regulatory T-Cells; Methods and Protocol Masahiro Ono Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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lymphoma

Phenotypic and Functional Studies of Human Treg Cell Subpopulationsohort, we now know that they have unprecedented underlying heterogeneity. This heterogeneity is reflected in their phenotype and functions. As human Treg subpopulations are very small in numbers, it is necessary to develop novel ways of isolating and manipulating these cell populations. In this chap

SEMI

In Vitro Analysis of CTLA-4-Mediated Transendocytosis by Regulatory T Cellscally, CTLA-4 on Tregs can attenuate T cell activation by physically removing and internalizing costimulatory ligands CD80 and CD86 from the surface of antigen-presenting cells by transendocytosis. Therefore, the process of transendocytosis can be harnessed as a tool to study the molecular basis of

發(fā)酵劑

Stricture

restrain

Regulatory T-Cell Phenotyping Using CyTOFtory T cell subsets is essential to understand their function. Mass cytometry or CyTOF is a technology that enables the simultaneous measurement of up to 50 markers in single cells by using antibodies tagged with heavy metals, which are then detected with time-of-flight mass spectrometry. This chapt

Spangle

Analysis of the In Vivo Function of Follicular Regulatory T (TFR) Cells in the Regulation of Antibodic assays that are able to precisely assess T. cell function, particularly its in vivo activity, independent of differentiation and other Tregs. Here we describe an adoptive transfer approach in conjugation with flow cytometry and ELISA to evaluate the T. cell function on T., B cells, and antibody response in vivo.

ATRIA

Analysis of Peripherally Derived Treg in the Intestine to analyze the interactions between the gut microbiota and pTreg are important for better understanding of the symbiotic relationship with these microorganisms. Here, we describe a protocol to isolate colonic lamina propria cells and analyze pTregs in mice.

DAUNT

Differentiation of Peripheral Tregery of ovalbumin into RAG-deficient DO11.10 mice, in which T cells are ovalbumin specific. We show the isolation of na?ve CD4. T cells by flow cytometry, the administration of ovalbumin in drinking water, and the analysis tools, including an optional protocol for the enrichment of analysis samples in CD4. T cells using a magnetic purification.

cocoon

Book 2023he topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols...?..Authoritative and cutting-edge,?.Regulatory T-Cells: Methods and Protocols?.aims?to be a?useful and practical guide to new researchers?and experts looking to expand their knowledge.?.