Titlebook: Regulatory B Cells; Methods and Protocol Francesca Mion,Silvia Tonon Book 2021Latest edition Springer Science+Business Media, LLC, part of

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Detection and Quantification of Transforming Growth Factor-β1 Produced by Murine B Cells: Pros and Cn determine the fate of naive T cells, such as differentiation, proliferation, and apoptosis, which are relevant to the pathogenesis of autoimmunity, infection, inflammation, allergy, and cancer. Here, we describe detailed methods for detection and quantification of TGF-β1 secreted by B cells using ELISA, flow cytometry, and real-time PCR.

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Regulatory B Cells978-1-0716-1237-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Purification and Immunophenotypic Characterization of Human CD19+CD24hiCD38hi and CD19+CD24hiCD27+ Bw to identify and isolate these subsets from peripheral blood B cells via flow cytometry. We also explain how to expand Bregs in culture and how to identify them based on intracellular expression of IL-10.

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Purification and Immune Phenotyping of B-1 Cells from Body Cavities of Miceith important functions in tissue homeostasis and immune defense. While B-1 cell frequencies in bone marrow and secondary lymphoid tissues are low, relative high frequencies exist within peritoneal and pleural cavities of mice, including both CD5. and CD5. B-1 cells. These cells represent B-1 reserv

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Purification and Immunophenotypic Characterization of Murine Plasma Cellsated cells sharing a unique phenotype. However, it lately became evident that ASCs exist in a variety of subsets differing by their lifespan, anatomic location, and immunological function. Thus, ASCs can exist as long-lived plasma cells (LLPC) that can persist for years in a nonproliferating state w

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