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Titlebook: Recombineering; Methods and Protocol Christopher R. Reisch Book 2022 Springer Science+Business Media, LLC, part of Springer Nature 2022 CRI

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發(fā)表于 2025-3-21 18:17:58 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Recombineering
副標(biāo)題Methods and Protocol
編輯Christopher R. Reisch
視頻videohttp://file.papertrans.cn/825/824114/824114.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Recombineering; Methods and Protocol Christopher R. Reisch Book 2022 Springer Science+Business Media, LLC, part of Springer Nature 2022 CRI
描述This volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Recombineering: Methods and Protocols. is a valuable resource for any researchers interested in learning more about this developing field.?.
出版日期Book 2022
關(guān)鍵詞CRISPR; DNA nucleases; genotyping; Escherichia coli; Gram-Negative Bacteria; Gram-Positive Bacteria; recom
版次1
doihttps://doi.org/10.1007/978-1-0716-2233-9
isbn_softcover978-1-0716-2235-3
isbn_ebook978-1-0716-2233-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2022
The information of publication is updating

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沙發(fā)
發(fā)表于 2025-3-21 21:31:25 | 只看該作者
Lambda Red Recombineering of Bacteriophage in the Lysogenic State,genome of a lysogenized host with a prophage compatible with bacteriophage lambda. Linear DNA is used as the recombination substrate and antibiotic resistance is used as the basis for selection of recombinants. The method enables the genetic manipulation of a prophage in 3–5?days.
板凳
發(fā)表于 2025-3-22 03:09:11 | 只看該作者
Recombineering-Mediated Genome Editing in Burkholderiales Strains, also closely related Gram-negative bacteria for efficient genome editing. However, some distant bacterial species like Burkholderiales strains require host-specific Redα/Redβ recombinase pair for highly efficient genome editing. A pair of recombinases Redαβ7029 from the Burkholderiales strain DSM 7
地板
發(fā)表于 2025-3-22 06:47:24 | 只看該作者
High-Efficiency Multi-site Genomic Editing (HEMSE) Made Easy,chnology allows to introduce mutations within bacterial genomes in a very simple and straightforward way. This technology was initially developed for . but was later extended to other organisms of interest, including the environmentally and metabolically versatile .. The technology is based on three
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發(fā)表于 2025-3-22 13:10:27 | 只看該作者
Plant Gene Modification by BAC Recombineering,rried by bacterial artificial chromosomes (BACs) in .. Whereas BAC transformation provides a simple means for integration of modified genes into the genomes of animal cells to generate knock-in and knockout lines, successful application of this strategy is hampered by low frequency of homologous rec
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發(fā)表于 2025-3-22 18:56:02 | 只看該作者
Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling, (DIRex). Dup-In is used for transferring existing mutations between strains, and DIRex for generating almost any type of mutation. Both techniques use intermediate insertions with counter-selectable cassettes, flanked by directly repeated sequences that enable exact and spontaneous excision of the
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發(fā)表于 2025-3-23 01:13:10 | 只看該作者
Genome Editing in , Using CRISPR/Cas9 Technology,ave recently developed a CRISPR/Cas9-based genome editing system (pCasKP-pSGKP) by coupling a CRISPR/Cas9 system with the lambda Red recombination system as well as a cytidine deaminase-mediated base editing system (pBECKP) in ., enabling rapid, scarless, and efficient genetic manipulation in divers
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