Titlebook: Recombinant Proteins from Plants; Methods and Protocol Jacqueline MacDonald,Igor Kolotilin,Rima Menassa Book 2016Latest edition Springer Sc

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Virus-Derived Vectors for the Expression of Multiple Proteins in Plantspression, an expression system based on translational enhancement by the untranslated regions of RNA-2 from cowpea mosaic virus (CPMV), named CPMV-., is used. The expression vector pEAQ-. combines the user-friendly pEAQ plasmid with CPMV-. to provide a system for high-level expression of proteins in plants.

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Transient Protein Expression by Agroinfiltration in Lettuceor pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.

HEW

Total Soluble Protein Extraction for Improved Proteomic Analysis of Transgenic Rice Plant Roots that include root tissue. For protein/proteome analysis of transgenic plant roots or of seedlings, which include root tissue, such pronounced protein degradation is especially undesirable. A facile protein extraction protocol is presented, which ensures that despite the inclusion of root tissues there is minimal loss in total protein components.

Ischemic-Stroke

Temporary Immersion Bioreactors for the Contained Production of Recombinant Proteins in Transplastom propagation of plastid or nuclear transformants, though is especially suitable for transplastomic biomass, as organogenesis leads to greater expression and accumulation of transplastomic proteins due to increases in chloroplast number and size.

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Mechanics

Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture Extractwever, this can be avoided by incorporating continuous systems, which are often preferred by the processing industry. This method chapter illustrates the capture of GFP-HFBI hydrophobin fusion protein from BY-2 plant cell suspension extract using a semi-continuous ATPS method.