Titlebook: Recombinant Protein Production in Yeast; Brigitte Gasser,Diethard Mattanovich Book 2019 Springer Science+Business Media, LLC, part of Spri

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1064-3745 tion advice from the experts.This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like partic

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Established and Upcoming Yeast Expression Systems,rical use, vast amount of data, and experience paved the way for . as a first yeast cell factory, and still it is an important expression platform as being the production host for several large volume products. Continuing special needs of each targeted product and different requirements of bioproces

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Selection of the Optimal Yeast Host for the Synthesis of Recombinant Enzymes,ndatory to obtain full functionality. The wide-range transformation/expression platform presented in this chapter can be used to select the optimal yeast host for high-level synthesis of the desired enzyme with favorable biochemical properties. This platform is composed of a selection marker and up

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Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in ,rapeutic proteins. Despite favorable characteristics of the .-based platform for application to heterologous gene expression, several problems and limitations, such as over-glycosylation and proteolytic degradation, can be encountered in the development of production strains for secretory proteins.

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Selection of Heterologous Protein-Producing Strains in ,ted for this species have been developed, ranging from the simple cloning of expression vectors to recently developed high-throughput methodologies using efficient cloning and assembly such as Gateway and Golden Gate strategies. The latter strategies, due to their modular character, enable multiple

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High-Throughput Screening and Selection of , Strains,uction capabilities for all assessed transformants. As clonal variation in such experiments is caused by diverging numbers and possibly also genomic locations of integrated (linearized) expression constructs, the productivity assessment of a larger number of strains is mandatory for selecting a set

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CRISPR/Cas9-Mediated Homology-Directed Genome Editing in , deletion of host genes. For efficient gene deletion, methods such as the split-marker technique have been established. However, synthetic biology trends move toward building up large and complex reaction networks, which often require endogenous gene knockouts and simultaneous overexpression of indi

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