Titlebook: Recombinant Protein Production in Yeast; Brigitte Gasser,Diethard Mattanovich Book 2019 Springer Science+Business Media, LLC, part of Spri

Monolithic

Thomas Gassler,Lina Heistinger,Diethard Mattanovich,Brigitte Gasser,Roland Prielhofer

玷污

Mari A. Piirainen,Heidi Salminen,Alexander D. Frey

keloid

Anita Emmerstorfer-Augustin,Tamara Wriessnegger,Melanie Hirz,Guenther Zellnig,Harald Pichler

健談

VOC

認(rèn)為

Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in ,itations, such as over-glycosylation and proteolytic degradation, can be encountered in the development of production strains for secretory proteins. Here, . genetic tools and host strains, developed for authentic processing and modification of secretory recombinant proteins, are introduced with the analytical protocols.

Suppository

Book 2019bleshooting and avoiding known pitfalls..Authoritative and cutting-edge,?.Recombinant Protein Production in Yeast:?Methods and Protocols.?aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.?.

面包屑

enfeeble

Selection of Heterologous Protein-Producing Strains in ,Here, we present the Golden Gate cloning strategy for the construction of multiple expression cassettes, the transformation into ., and the selection of efficient enzyme-producing strains using an insect alpha-amylase as a reporter detected via a?thermal cycler-based microassay.

和諧

Assessing the Quality of Recombinant Products Made in Yeast,determination of posttranslational modifications such as glycan analysis are not described. In this regard, chromatographic, high-resolution technologies for the integrity of proteins as well as Western blot with specific detection methods are introduced, and individual strengths and perceived limitations are highlighted.