找回密碼
 To register

QQ登錄

只需一步,快速開始

掃一掃,訪問微社區(qū)

打印 上一主題 下一主題

Titlebook: Recombinant Protein Expression in Mammalian Cells; Methods and Protocol David L. Hacker Book 2018 Springer Science+Business Media, LLC, par

[復(fù)制鏈接]
查看: 26328|回復(fù): 63
樓主
發(fā)表于 2025-3-21 19:19:00 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Recombinant Protein Expression in Mammalian Cells
副標題Methods and Protocol
編輯David L. Hacker
視頻videohttp://file.papertrans.cn/825/824099/824099.mp4
概述Includes cutting-edge methods and protocols for the study of recombinant protein expression.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Recombinant Protein Expression in Mammalian Cells; Methods and Protocol David L. Hacker Book 2018 Springer Science+Business Media, LLC, par
描述.This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host;? however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and convenient, .Recombinant Protein Expression in Mammalian Cells: Methods and Protocols. aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins..
出版日期Book 2018
關(guān)鍵詞Transient protein production; Recombinant cell lines; Chinese hamster ovary cells; Human embryonic kidn
版次1
doihttps://doi.org/10.1007/978-1-4939-8730-6
isbn_softcover978-1-4939-9377-2
isbn_ebook978-1-4939-8730-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
The information of publication is updating

書目名稱Recombinant Protein Expression in Mammalian Cells影響因子(影響力)




書目名稱Recombinant Protein Expression in Mammalian Cells影響因子(影響力)學(xué)科排名




書目名稱Recombinant Protein Expression in Mammalian Cells網(wǎng)絡(luò)公開度




書目名稱Recombinant Protein Expression in Mammalian Cells網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Recombinant Protein Expression in Mammalian Cells被引頻次




書目名稱Recombinant Protein Expression in Mammalian Cells被引頻次學(xué)科排名




書目名稱Recombinant Protein Expression in Mammalian Cells年度引用




書目名稱Recombinant Protein Expression in Mammalian Cells年度引用學(xué)科排名




書目名稱Recombinant Protein Expression in Mammalian Cells讀者反饋




書目名稱Recombinant Protein Expression in Mammalian Cells讀者反饋學(xué)科排名




單選投票, 共有 1 人參與投票
 

1票 100.00%

Perfect with Aesthetics

 

0票 0.00%

Better Implies Difficulty

 

0票 0.00%

Good and Satisfactory

 

0票 0.00%

Adverse Performance

 

0票 0.00%

Disdainful Garbage

您所在的用戶組沒有投票權(quán)限
沙發(fā)
發(fā)表于 2025-3-22 00:16:31 | 只看該作者
Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHse hamster ovary (CHO-DG44) cells. These operations include bioreactor setup and configuration, batching media, inoculation of the seed cell culture, production phase, and harvest of cell-free fluids.
板凳
發(fā)表于 2025-3-22 04:02:56 | 只看該作者
地板
發(fā)表于 2025-3-22 06:37:11 | 只看該作者
Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV),ss, cell cultures are further processed using different methods for lysis and recovery. Methods for the purification of viral particles are described, including iodixanol gradient purification, immunoaffinity chromatography, and ultrafiltration, as well as quantitative PCR to quantify vector titer.
5#
發(fā)表于 2025-3-22 09:55:11 | 只看該作者
6#
發(fā)表于 2025-3-22 15:08:22 | 只看該作者
7#
發(fā)表于 2025-3-22 17:12:48 | 只看該作者
8#
發(fā)表于 2025-3-22 23:19:39 | 只看該作者
9#
發(fā)表于 2025-3-23 02:42:44 | 只看該作者
10#
發(fā)表于 2025-3-23 06:52:14 | 只看該作者
 關(guān)于派博傳思  派博傳思旗下網(wǎng)站  友情鏈接
派博傳思介紹 公司地理位置 論文服務(wù)流程 影響因子官網(wǎng) 吾愛論文網(wǎng) 大講堂 北京大學(xué) Oxford Uni. Harvard Uni.
發(fā)展歷史沿革 期刊點評 投稿經(jīng)驗總結(jié) SCIENCEGARD IMPACTFACTOR 派博系數(shù) 清華大學(xué) Yale Uni. Stanford Uni.
QQ|Archiver|手機版|小黑屋| 派博傳思國際 ( 京公網(wǎng)安備110108008328) GMT+8, 2025-10-13 18:59
Copyright © 2001-2015 派博傳思   京公網(wǎng)安備110108008328 版權(quán)所有 All rights reserved
快速回復(fù) 返回頂部 返回列表
临桂县| 黄龙县| 泗水县| 平湖市| 康马县| 六枝特区| 哈巴河县| 乃东县| 西安市| 丽水市| 沧州市| 鲁山县| 乌拉特中旗| 维西| 石棉县| 梧州市| 巴楚县| 汉寿县| 平遥县| 自贡市| 葫芦岛市| 甘谷县| 荥阳市| 乌兰县| 确山县| 山阴县| 双牌县| 客服| 南乐县| 梓潼县| 满洲里市| 江孜县| 华坪县| 普兰店市| 鱼台县| 邢台市| 洛阳市| 明光市| 茶陵县| 西充县| 来凤县|