Titlebook: Recombinant Glycoprotein Production; Methods and Protocol Virgínia Pican?o-Castro,Kamilla Swiech Book 2018 Springer Science+Business Media,

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書目名稱Recombinant Glycoprotein Production影響因子(影響力)




書目名稱Recombinant Glycoprotein Production影響因子(影響力)學科排名




書目名稱Recombinant Glycoprotein Production網(wǎng)絡公開度




書目名稱Recombinant Glycoprotein Production網(wǎng)絡公開度學科排名




書目名稱Recombinant Glycoprotein Production被引頻次




書目名稱Recombinant Glycoprotein Production被引頻次學科排名




書目名稱Recombinant Glycoprotein Production年度引用




書目名稱Recombinant Glycoprotein Production年度引用學科排名




書目名稱Recombinant Glycoprotein Production讀者反饋




書目名稱Recombinant Glycoprotein Production讀者反饋學科排名

諂媚于人

BAIL

Affection

Platforms for Recombinant Therapeutic Glycoprotein Production,in production, since glycosylation influences yield, pharmacokinetics, biological activity, and immunogenicity. This chapter covers the general aspects of therapeutic recombinant glycoproteins and the platforms that are being employed for their production.

雀斑

Purification Methods for Recombinant Factor VIII Expressed in Human Liver SK-Hep Cellsnity FVIII select and ion exchange SP-Fastflow chromatography steps without subjecting the target molecule to mechanical and temperature stress, separating impurities from rFVIII using net charge, hydrophobicity, and affinity of the molecules.

捕鯨魚叉

使?jié)M足

Human Cells as Platform to Produce Gamma-Carboxylated Proteinsthe methods to generate human cells as a platform to produce gamma-carboxylated proteins, for example the coagulation factors VII and IX, are presented. From the cell line modification up to the vitamin K adaptation of the produced cells is described in the protocols presented in this chapter.

背信

Production of Recombinant Factor VIII in Human Cell Linesherefore, the production of recombinant factor VIII (rFVIII) in human cell lines is a new approach to avoid nonhuman glycosylation. Here, we describe a protocol to produce rFVIII in the human cell line SK-HEP, using a lentiviral vector to produce high quantities of the recombinant protein.

嫻熟

Strategies to Suspension Serum-Free Adaptation of Mammalian Cell Lines for Recombinant Glycoprotein s is challenging, time-consuming, and cell line and medium dependent. In this chapter, we present different approaches that can be used to adapt mammalian cell lines from an anchorage-dependent serum supplemented culture to a suspension serum-free culture.

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