Titlebook: Recombinant Gene Expression; Argelia Lorence Book 2012Latest edition Springer Science+Business Media, LLC 2012 Bacteria.Bacteria.Recombina

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書目名稱Recombinant Gene Expression影響因子(影響力)




書目名稱Recombinant Gene Expression影響因子(影響力)學(xué)科排名




書目名稱Recombinant Gene Expression網(wǎng)絡(luò)公開度




書目名稱Recombinant Gene Expression網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Recombinant Gene Expression被引頻次




書目名稱Recombinant Gene Expression被引頻次學(xué)科排名




書目名稱Recombinant Gene Expression年度引用




書目名稱Recombinant Gene Expression年度引用學(xué)科排名




書目名稱Recombinant Gene Expression讀者反饋




書目名稱Recombinant Gene Expression讀者反饋學(xué)科排名

新鮮

A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein–ProtHEX, which is compatible, and thus can be partnered, with many commercially available . vectors, such as pGEX and pMAL. Either of the vectors allows proteins to be expressed individually as a tagged fusion protein and can be used directly for protein co-purification. This protocol presents the experimental procedure for the co-expression method.

獨(dú)行者

Recombinant Protein Production in the Eukaryotic Protozoan Parasite ,: A Reviewmammalian-type posttranslational modification of target proteins. Although there are few examples of recombinant protein expression using this system, it can be an attractive alternative to using mammalian cells. This chapter presents an overview of the newly developed protein expression system based on ..

得意人

靦腆

Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass Spectrometry direct means to unequivocally confirm identity of recombinant proteins based on predicted peptide profiles. Many universities or research institutions now carry mass spectrometry instrumentation as part of their core bioanalytical facilities or provide public service to outside investigators. This

incision

A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene Expression genes. Genome-wide chromatin analyses have shown that there are common chromatin organization patterns for most genes but have also revealed important differences in nucleosome positioning throughout the genome. Such chromatin heterogeneity is one of the reasons why recombinant gene expression is h

吃掉

Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant Proteinsrtant and widely used antibody-based procedures for recombinant protein applications are Western immunoblotting and enzyme-linked immunosorbent assays (ELISAs). These analyses require well-characterized, sensitive, and high-affinity antibodies that specifically and selectively recognize the recombin

Euphonious

關(guān)心

Formidable

Optimization of Purification Protocols Based on the Step-by-Step Monitoring of the Protein Aggregatee aggregates of different complexity widely represented in such fractions. The use of fusing target protein domains to highly soluble carriers may strongly contribute to soluble aggregate accumulation. Therefore, reliable analytical methods must be used to evaluate the biophysical characteristics of