Titlebook: Recombinant Enzymes - From Basic Science to Commercialization; Azura Amid Book 2015 Springer International Publishing Switzerland 2015 bro

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Common Laboratory Procedure, The principle and theory behind each experiment is presented to help the reader understand each step that is conducted in an experiment so the reader will be able to select and modify the procedures according to the type of enzyme they are working with. Examples of procedures and the results obtain

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Characterization of Recombinant Enzymes,ies but the spectrum of methods and the extent of characterization are related to the end use of the enzyme. Understanding the biochemical properties and mechanisms of enzymes are essential for research and development as well as for enzyme commercialization. These include among others the establish

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Purification of Recombinant Protein for Industrial Use,eview briefly describes the choices of expression systems available to produce recombinant protein and based on the choices made, techniques used for sample harvesting is deliberated. The method to prepare samples prior to the purification step is also discussed which includes clarification and reta

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Downstream Processing of Recombinant Enzymes for Commercialization,nzyme and of higher purity. The commercialized processes involved should be optimized for higher yield and quality and at the same time adopt a simplified and cost effective processing systems. Once the manufacturing facility being endorsed by the regulatory authorities, the operation must adhere to

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aspersion

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Case Study: Recombinant Bromelain Cloning, Characterization and Upstream Processes,rocedures had patents filed in Malaysia, Europe and the United State of America with application numbers PI 20095434, 10015711.4, and 12968766, respectively. Portions of the experimental procedures were also presented in our publication (Amid et al., Process Biochem 46:2232–2239, 2011). The entire c

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Book 2015hers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product..Readers will discover the impor