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Titlebook: Receptor Binding Techniques; Mary Keen Book 19991st edition Humana Press 1999

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發(fā)表于 2025-3-21 17:02:46 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Receptor Binding Techniques
編輯Mary Keen
視頻videohttp://file.papertrans.cn/824/823544/823544.mp4
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Receptor Binding Techniques;  Mary Keen Book 19991st edition Humana Press 1999
描述Radioligand binding is essentially a very simple technique, applicable to a wide range of preparations. It can of course be used to study many diff- ent types of receptors, but can also be adapted to investigate a variety of nonreceptor proteins, such as enzymes and uptake sites. The basic technique allows the affinity of drugs for the receptor of interest to be determined very easily, and the speed and precision of binding have led to its widespread use as a primary screen in drug discovery programs. It also allows receptor n- ber to be measured and the kinetics of the receptor-ligand interaction to be determined, parameters not readily accessible by any other means. Receptor distribution can be studied in great detail using the technique of autoradiog- phy; radioligand binding provides the most sensitive technique for dissecting allosteric interactions between ligands and receptors, and even between rec- tors and effector molecules, such as G proteins. Moreover, by allowing rec- tors to be identified in the absence of any measurable response, binding is a prerequisite for receptor solubilization and purification studies. It is even p- sible to measure ligand receptor interactions
出版日期Book 19991st edition
版次1
doihttps://doi.org/10.1385/0896035301
isbn_ebook978-1-59259-579-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1999
The information of publication is updating

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Solubilized Muscarinic Acetylcholine Receptors from the Rat Myocardium: Pharmacological and Hydrodynomatography in the purification procedure. Furthermore, investigation of the pharmacological and hydrodynamic characteristics of solubilized receptors can, in itself, yield useful information about receptor function, particularly regarding the role of accessory proteins and/or membrane lipids in ligand binding.
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Definition of Receptors in Radioligand-Binding Experiments receptors is by no means a simple problem and is one that has dogged binding studies from their inception. The essence of the problem is this: the definition of a receptor is functional—drugs bind to receptors and produce a response. However, no response is measured in binding experiments.
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Whole Human Brain Autoradiography of Serotonin Re-Uptake Inhibitorson Emission Tomography (PET) scanning. The effects of unilateral lesion on receptors in the adjacent hemisphere may be determined, and the distribution of receptor sites affected by disease states can be mapped across the width of the brain to see if there is an asymmetrical pattern of receptor modification or loss.
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[3H]-GABA Binding to GABAA and GABAB Sites on Rat Brain Crude Synaptic Membraness: GABA., GABA., and GABA.. GABA. receptors are pharmacologically defined on the basis of antagonism by bicuculline and insensitivity to the GABA-analog baclofen, whereas GABA. receptors are stimulated by baclofen and are insensitive to bicuculline; GABA. receptors are insensitive to both (.).
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