Titlebook: Real-Time Analysis of Biomolecular Interactions; Applications of BIAC Kazuhiro Nagata,Hiroshi Handa Book 2000 Springer Japan 2000 biologica

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雜色

是剝皮

CHARM

Principles of Surface Plasmon Resonancee to the surface of a sensor (up to a istance of about 500 nm from the surface). Binding of molecules to the surface as a result of biospecific interaction changes the solute concentration in the surface volume, which influences the refractive index. The resulting change in the SPR signal is followe

斜谷

Principles of BIACOREt labeling. One of the interacting partners is immobilized on the surface of a sensor chip, while the sample containing the other partner(s) is injected over the surface at a constant flow rate through a microfluidic channel system. The minute changes in mass concentrations at the surface of the sen

Bombast

Development of BIACOREan genome project (HUGO) and other large-scale genetic information analysis projects, have contributed to the discovery of many new proteins, and there is a growing demand for analysis of the interaction properties of these novel biomolecules. A detailed quantitative description of the recognition a

perpetual

Using BIACORE as Pots and Pansecking the SDS-PAGE gave evidence of GST fusion protein produced in large amount, but its band was not detected in the sample of supernatant after centrifugation. It was apparent that the recombinant had formed inclusion bodies. I am sure many of you often have the same experiences.

CLOWN

NAVEN

Using BIACORE as Pots and Panscause all of it had gone into inclusion bodies. It did not exhibit any activity, either, even after the pellet was solubilized with urea. Judging from the amino acid sequences deduced from cDNA, this protein has 10 cysteine residues and seems to have complicated disulphide bridges. We were puzzled w

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