Titlebook: Rapid Methods for Analysis of Biological Materials in the Environment; Peter J. Stopa,Michael A. Bartoszcze Book 2000 Springer Science+Bus

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Field Applications of Flow Cytometry,biological, and/or chemical properties. Over the past several years, we have investigated the use of flow cytometry to detect and identify microorganisms in aerosols. The effort to date has shown that changes in scatter patterns or DNA composition suggest that changes in the ambient air are evident.

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Biodiversity of Bacterial Populations Monitored by Flow Cytometry,contents of the cells were measured by FACStarPlus flow cytofluotometer (Becton Dickinson). The bacterial neuston contains more cells from the beginning stage of cell division cycle and DNA replication cycle that the cells from the bulk of water. Anabiotic and endotrophic subpopulations of bacilli a

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Measurement of Biological Aerosol with a Fluorescent Aerodynamic Particle Sizer (Flaps): Correlatioapabilities will be useful in environmental monitoring, for example, in gathering information in perceived hazardous areas like housing developments downwind of sewage treatment plants..Experience gained from measuring fluorescence signals of single bacterial spores under flow cytometry using UV exc

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Development of PCR-Based Assays for the Detection and Molecular Genotyping of Microorganisms of Imp Several different strategies can be used for identifying and cloning unique nucleic acid sequences which can detect one type of microorganism from others found in mixed samples or the environment. Specific PCR probes can be obtained from plasm id and chromosomal libraries, ribosomal DNA genes, rep-

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Use of Taqman, Light Cycler, and Confocal Microscropy to Detect Specific PCR,tion of specific DNA sequences and a fluorogenic probe tat contains both a reporter and quencher dye. During amplification the probe hybridizes with a target on the amplicon, and the 5′-exonuclease activity of DNA polymerase hydrolyzes the probe. The separation of the reporter from the quencher resu

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Robotics Mission to Mars from Exobiological Point of View,ological activity was due to the presence of a thick atmosphere and the resulting warmer temperature. From a biological perspective the existence of liquid water, by itself motivates the question of the origin of life on Mars.