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Titlebook: Rab GTPases; Methods and Protocol Guangpu Li Book 2015 Springer Science+Business Media New York 2015 Endocytic Pathways.Eukaryotes.Exocytic

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書目名稱Rab GTPases
副標題Methods and Protocol
編輯Guangpu Li
視頻videohttp://file.papertrans.cn/821/820250/820250.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Rab GTPases; Methods and Protocol Guangpu Li Book 2015 Springer Science+Business Media New York 2015 Endocytic Pathways.Eukaryotes.Exocytic
描述.This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters and starts with an overview of the Rab GTPase family. The next few chapters describe systematic approaches to the identification and classification of Rabs and Rab GAPs, as well as the detection of Rab isoprenylation and membrane distribution. The last few chapters examine the biochemical and functional properties of individual Rabs in the order of exocytic, recycling and endocytic Rabs. Written in the highly successful?.Methods of Molecular Biology.?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls..Instructive and practical,?.Rab GTPases: Methods and Protocols.?approaches each topic with great detail and is a valuable resource for researchers and students interested in the field of Rab GTPases..
出版日期Book 2015
關(guān)鍵詞Endocytic Pathways; Eukaryotes; Exocytic Pathways; GTPase-Activating Proteins; Guanine Nucleotide Exchan
版次1
doihttps://doi.org/10.1007/978-1-4939-2569-8
isbn_softcover978-1-4939-5594-7
isbn_ebook978-1-4939-2569-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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Bioinformatic Approaches to Identifying and Classifying Rab Proteins,cularly challenging for standard annotation pipelines because they are similar to other small GTPases and form a large family with many paralogous subfamilies. Here, we describe a bioinformatic annotation pipeline specifically tailored to Rab GTPases. It proceeds in two steps: first, Rabs are distin
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High-Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins,unction of GTPases in diverse cellular processes. For the Rab GTPase family, which comprises at least 60 distinct proteins in humans, putative GAPs have been identified from both eukaryotic organisms and pathogenic bacteria. A major obstacle has involved identification of target substrates and deter
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