| 書目名稱 | RT-PCR Protocols | | 副標(biāo)題 | Second Edition | | 編輯 | Nicola King | | 視頻video | http://file.papertrans.cn/821/820243/820243.mp4 | | 概述 | Features all the latest and hottest technology used in conventional and real-time RT-PCR.Provides a broad scope covered in good depth, from detection of minute qualities of microRNA to the analysis of | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | .Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In .RT-PCR Protocols, Second Edition., expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful .Methods in Molecular Biology.? series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls...User friendly and up-to-date, .RT-PCR Protocols, Second Edition. acts as a handy companion to scientists from numerous diverse backgrounds who wish to | | 出版日期 | Book 2010Latest edition | | 關(guān)鍵詞 | Cloning; Gene sequences; PCR; Primer design; Real-time quantitative RT-PCR; Reverse transcription; Reverse | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-60761-629-0 | | isbn_softcover | 978-1-4939-5682-1 | | isbn_ebook | 978-1-60761-629-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC 2010 |
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